Measurements of Hyperproteinaemia in Human Blood Using Laser Interferometry: In Vitro Study
H. Ullah, A. Ghalib, M. Akhtar, R. Mehmood, A. Akram, Z. Batool, A. Nazir, G. Gilanie and S. Habib
Blood consists of many numerous cells and presence of protein makes it thicker than water. According to medical research, a normal person has in excess of a 5 l of blood. Protein is natural compound which is naturally found in the human body. In this study, the absorbance of human blood in the range of ultraviolet (UV) and visible (VIS), from 190 to 1100 nm was measured. In our experimental work the absorbance peaks and transmittance peaks have been observed to agree with reported literature values. We have also studied the behaviour of human blood in region of near infrared and calculated the contents of protein with the help of spectrophotometric method by measuring the absorbance value in the test serum with enzymatic reagents. The qualitative measurement of all the samples were measure using dark field microscope with a laser wavelength of 630 nm and bandwidth of 15 nm. In this work we have also established the relation between the concentrations of total protein level with the complete blood count.
Keywords: He-Ne laser, Hyperproteinaemia, enzymatic reagents, spectroscopy, optical microscopy ultraviolet (UV), visible (VIS), in vitro