Proteins and Triglycerides Measurement in Blood Under Ultraviolet (UV)/Visible (Vis) Spectroscopy at λ=190 to 1100 nm with an Additional He-Ne Laser Source
H. Ullah, Z. Batool, A. Nazir, U. Akhtar, G. Gilanie, J. Rehman, N. Amin and S. Habib
In this work the absorbance of human blood in the range of the ultraviolet (UV) and visible (Vis) regions was analysed with a UV/Vis spectrometer with He-Ne laser illumination for alignment. We have also studied the behavior of human blood in some regions of the near infrared (NIR). We have determined the contents of protein and triglycerides with help of spectrophotometric method by measuring the absorbance values in test serum with enzymatic reagents. The aforementioned work has correlated the concentration of total proteins and triglycerides level with complete blood count (CBC).
Keywords: He-Ne laser, human blood, spectrophotometry, proteins, triglycerides, complete blood count (CBC), optical properties