Identification of Liposarcoma from Biopsy of the Abdomen Using Microscopy with a Diode Laser Light Source at a Wavelength of 630 nm and an Ultraviolet (UV)/Visible Spectrophotometer in the Wavelength Range of 190 to 1100 nm
H. Ullah, M. Rizwan A. Nazir and Z. Batool
The objectives of this work were to image the liposarcoma with light microscopy and measure the absorbance spectra of liposarcoma and different mammalian blood in range of ultraviolet (UV), visible region and infrared (IR) range. We have made the systematic analysis of mammalian blood of species with a high (human), medium (cow) and low (goat) of absorbance. A customized optical diode laser light source microscope (laser wavelength, λo=630 nm and bandwidth, Δλ=15 nm) and an UV/ visible spectrophotometer (wavelength, λ=190 to 1100 nm) have been used for investigation of well differentiated liposarcoma in abdomen tis- sues and mammalian blood. We have observed that the shapes and size of the cells are not uniform throughout the cancerous tissue. The marks on the cells and dead cells were also there in plenty. Cellular spaces between the cells have also been observed. We have seen that the shapes and size of the cells shows unpredictability.
Keywords: Diode laser, laser beam, whole blood, liposarcoma, human, cow, goat, spectrophotometer, microscopy, two-dimensional (2-D) imaging